version 2014a Search Results


matlab version 2014a  (MathWorks Inc)
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Matlab Version 2014a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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version 2014a  (MathWorks Inc)
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MathWorks Inc version 2014a
Version 2014a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab code 2014a  (MathWorks Inc)
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Matlab Code 2014a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chemometricks toolbox  (MathWorks Inc)
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gaussian mixture distribution model  (MathWorks Inc)
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stata version 13.1  (STATA Corporation)
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matlab 2015a  (MathWorks Inc)
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Matlab 2015a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2014a (version 8.3.0.532)  (MathWorks Inc)
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2014a (Version 8.3.0.532), supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scils lab  (Bruker Corporation)
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Bruker Corporation scils lab
<t>MALDI</t> imaging MS of N -linked glycans released from formalin-fixed murine kidney sections. Formalin-fixed murine kidney sections were treated with antigen retrieval prior to printing of 30 nL/spot dialyzed PNGase F or buffer control arrays with 250 μm spacing. 2,5-DHB (20 mg/mL) was sprayed onto the sections and MS spectra were acquired by oversampling at 100 μm intervals using a MALDI-TOF/TOF MS instrument. Data was loaded raw into <t>SCiLS,</t> preprocessed for baseline subtraction and normalization to total ion current (TIC) prior to segmentation analysis (maximum processing mode, interval width of 0.5 Da, strong smoothing) and pLSA (10 component, interval width of 0.5 Da, random initiation). Regions are outlined for PNGase F regions 1 ( red ) and 2 ( blue ), as well as control ( green ) and calibrant ( small red box ) regions. a The segmentation map for the entire data set, which discriminates between the cortex and medulla/pelvis of the kidney at the segmentation levels selected (see inset ). b – d Components 1, 2, and 8 from the pLSA analysis, which also discriminate the same regions of the tissue. e – h Ion intensity maps for (Hex) 2 (HexNAc) 2 + (Man) 3 (GlcNAc) 2 ( m / z 1663.632), (Hex) 6 + (Man) 3 (GlcNAc) 2 ( m / z 1905.697), (Hex)2(HexNAc) 3 (Deoxyhexose) 3 + (Man) 3 (GlcNAc) 2 ( m / z 2304.909), and (Hex) 3 (HexNAc) 4 (Deoxyhexose) 4 + (Man) 3 (GlcNAc) 2 ( m / z 2816.115). Intensity scales are autocorrected for these intensity maps (with weak denoising) and scale bars are included
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winfolia software  (Regent Instruments)
90
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<t>MALDI</t> imaging MS of N -linked glycans released from formalin-fixed murine kidney sections. Formalin-fixed murine kidney sections were treated with antigen retrieval prior to printing of 30 nL/spot dialyzed PNGase F or buffer control arrays with 250 μm spacing. 2,5-DHB (20 mg/mL) was sprayed onto the sections and MS spectra were acquired by oversampling at 100 μm intervals using a MALDI-TOF/TOF MS instrument. Data was loaded raw into <t>SCiLS,</t> preprocessed for baseline subtraction and normalization to total ion current (TIC) prior to segmentation analysis (maximum processing mode, interval width of 0.5 Da, strong smoothing) and pLSA (10 component, interval width of 0.5 Da, random initiation). Regions are outlined for PNGase F regions 1 ( red ) and 2 ( blue ), as well as control ( green ) and calibrant ( small red box ) regions. a The segmentation map for the entire data set, which discriminates between the cortex and medulla/pelvis of the kidney at the segmentation levels selected (see inset ). b – d Components 1, 2, and 8 from the pLSA analysis, which also discriminate the same regions of the tissue. e – h Ion intensity maps for (Hex) 2 (HexNAc) 2 + (Man) 3 (GlcNAc) 2 ( m / z 1663.632), (Hex) 6 + (Man) 3 (GlcNAc) 2 ( m / z 1905.697), (Hex)2(HexNAc) 3 (Deoxyhexose) 3 + (Man) 3 (GlcNAc) 2 ( m / z 2304.909), and (Hex) 3 (HexNAc) 4 (Deoxyhexose) 4 + (Man) 3 (GlcNAc) 2 ( m / z 2816.115). Intensity scales are autocorrected for these intensity maps (with weak denoising) and scale bars are included
Winfolia Software, supplied by Regent Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matlab 2012a  (MathWorks Inc)
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<t>MALDI</t> imaging MS of N -linked glycans released from formalin-fixed murine kidney sections. Formalin-fixed murine kidney sections were treated with antigen retrieval prior to printing of 30 nL/spot dialyzed PNGase F or buffer control arrays with 250 μm spacing. 2,5-DHB (20 mg/mL) was sprayed onto the sections and MS spectra were acquired by oversampling at 100 μm intervals using a MALDI-TOF/TOF MS instrument. Data was loaded raw into <t>SCiLS,</t> preprocessed for baseline subtraction and normalization to total ion current (TIC) prior to segmentation analysis (maximum processing mode, interval width of 0.5 Da, strong smoothing) and pLSA (10 component, interval width of 0.5 Da, random initiation). Regions are outlined for PNGase F regions 1 ( red ) and 2 ( blue ), as well as control ( green ) and calibrant ( small red box ) regions. a The segmentation map for the entire data set, which discriminates between the cortex and medulla/pelvis of the kidney at the segmentation levels selected (see inset ). b – d Components 1, 2, and 8 from the pLSA analysis, which also discriminate the same regions of the tissue. e – h Ion intensity maps for (Hex) 2 (HexNAc) 2 + (Man) 3 (GlcNAc) 2 ( m / z 1663.632), (Hex) 6 + (Man) 3 (GlcNAc) 2 ( m / z 1905.697), (Hex)2(HexNAc) 3 (Deoxyhexose) 3 + (Man) 3 (GlcNAc) 2 ( m / z 2304.909), and (Hex) 3 (HexNAc) 4 (Deoxyhexose) 4 + (Man) 3 (GlcNAc) 2 ( m / z 2816.115). Intensity scales are autocorrected for these intensity maps (with weak denoising) and scale bars are included
Matlab 2012a, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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16-slice helical ct somatom sensation 64  (Siemens Healthineers)
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Siemens Healthineers 16-slice helical ct somatom sensation 64
<t>MALDI</t> imaging MS of N -linked glycans released from formalin-fixed murine kidney sections. Formalin-fixed murine kidney sections were treated with antigen retrieval prior to printing of 30 nL/spot dialyzed PNGase F or buffer control arrays with 250 μm spacing. 2,5-DHB (20 mg/mL) was sprayed onto the sections and MS spectra were acquired by oversampling at 100 μm intervals using a MALDI-TOF/TOF MS instrument. Data was loaded raw into <t>SCiLS,</t> preprocessed for baseline subtraction and normalization to total ion current (TIC) prior to segmentation analysis (maximum processing mode, interval width of 0.5 Da, strong smoothing) and pLSA (10 component, interval width of 0.5 Da, random initiation). Regions are outlined for PNGase F regions 1 ( red ) and 2 ( blue ), as well as control ( green ) and calibrant ( small red box ) regions. a The segmentation map for the entire data set, which discriminates between the cortex and medulla/pelvis of the kidney at the segmentation levels selected (see inset ). b – d Components 1, 2, and 8 from the pLSA analysis, which also discriminate the same regions of the tissue. e – h Ion intensity maps for (Hex) 2 (HexNAc) 2 + (Man) 3 (GlcNAc) 2 ( m / z 1663.632), (Hex) 6 + (Man) 3 (GlcNAc) 2 ( m / z 1905.697), (Hex)2(HexNAc) 3 (Deoxyhexose) 3 + (Man) 3 (GlcNAc) 2 ( m / z 2304.909), and (Hex) 3 (HexNAc) 4 (Deoxyhexose) 4 + (Man) 3 (GlcNAc) 2 ( m / z 2816.115). Intensity scales are autocorrected for these intensity maps (with weak denoising) and scale bars are included
16 Slice Helical Ct Somatom Sensation 64, supplied by Siemens Healthineers, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


MALDI imaging MS of N -linked glycans released from formalin-fixed murine kidney sections. Formalin-fixed murine kidney sections were treated with antigen retrieval prior to printing of 30 nL/spot dialyzed PNGase F or buffer control arrays with 250 μm spacing. 2,5-DHB (20 mg/mL) was sprayed onto the sections and MS spectra were acquired by oversampling at 100 μm intervals using a MALDI-TOF/TOF MS instrument. Data was loaded raw into SCiLS, preprocessed for baseline subtraction and normalization to total ion current (TIC) prior to segmentation analysis (maximum processing mode, interval width of 0.5 Da, strong smoothing) and pLSA (10 component, interval width of 0.5 Da, random initiation). Regions are outlined for PNGase F regions 1 ( red ) and 2 ( blue ), as well as control ( green ) and calibrant ( small red box ) regions. a The segmentation map for the entire data set, which discriminates between the cortex and medulla/pelvis of the kidney at the segmentation levels selected (see inset ). b – d Components 1, 2, and 8 from the pLSA analysis, which also discriminate the same regions of the tissue. e – h Ion intensity maps for (Hex) 2 (HexNAc) 2 + (Man) 3 (GlcNAc) 2 ( m / z 1663.632), (Hex) 6 + (Man) 3 (GlcNAc) 2 ( m / z 1905.697), (Hex)2(HexNAc) 3 (Deoxyhexose) 3 + (Man) 3 (GlcNAc) 2 ( m / z 2304.909), and (Hex) 3 (HexNAc) 4 (Deoxyhexose) 4 + (Man) 3 (GlcNAc) 2 ( m / z 2816.115). Intensity scales are autocorrected for these intensity maps (with weak denoising) and scale bars are included

Journal: Analytical and Bioanalytical Chemistry

Article Title: MALDI imaging mass spectrometry of N -linked glycans on formalin-fixed paraffin-embedded murine kidney

doi: 10.1007/s00216-014-8293-7

Figure Lengend Snippet: MALDI imaging MS of N -linked glycans released from formalin-fixed murine kidney sections. Formalin-fixed murine kidney sections were treated with antigen retrieval prior to printing of 30 nL/spot dialyzed PNGase F or buffer control arrays with 250 μm spacing. 2,5-DHB (20 mg/mL) was sprayed onto the sections and MS spectra were acquired by oversampling at 100 μm intervals using a MALDI-TOF/TOF MS instrument. Data was loaded raw into SCiLS, preprocessed for baseline subtraction and normalization to total ion current (TIC) prior to segmentation analysis (maximum processing mode, interval width of 0.5 Da, strong smoothing) and pLSA (10 component, interval width of 0.5 Da, random initiation). Regions are outlined for PNGase F regions 1 ( red ) and 2 ( blue ), as well as control ( green ) and calibrant ( small red box ) regions. a The segmentation map for the entire data set, which discriminates between the cortex and medulla/pelvis of the kidney at the segmentation levels selected (see inset ). b – d Components 1, 2, and 8 from the pLSA analysis, which also discriminate the same regions of the tissue. e – h Ion intensity maps for (Hex) 2 (HexNAc) 2 + (Man) 3 (GlcNAc) 2 ( m / z 1663.632), (Hex) 6 + (Man) 3 (GlcNAc) 2 ( m / z 1905.697), (Hex)2(HexNAc) 3 (Deoxyhexose) 3 + (Man) 3 (GlcNAc) 2 ( m / z 2304.909), and (Hex) 3 (HexNAc) 4 (Deoxyhexose) 4 + (Man) 3 (GlcNAc) 2 ( m / z 2816.115). Intensity scales are autocorrected for these intensity maps (with weak denoising) and scale bars are included

Article Snippet: Abundance weighted mean (AWM, weighted by S / N ) m / z was calculated for each of these peak groups. (ii) MALDI imaging: Data was analyzed by SCiLS Lab (version 2014a, SCiLS GmbH, Bruker Daltonics) or by exporting peak lists from flexAnalysis (TopHat baseline subtraction and SN >3) and in-house R [ ] processing for difference in proportions statistic (DIPPS) analysis.

Techniques: Imaging